The Exhaustive Photon Reassignment (EPR) algorithm (developed and patented by the Biomedical Imaging Center at the University of Massachusetts Medical School in Worcester), when applied to a through-focus stack of images acquired from any conventional fluorescence microscope or confocal micorscope, reassigns all out-of-focus photons back to their proper place of origin, producing very "thin" optical sections. These images which are of very high contrast, reveal fine small structures which are not visible in the unprocessed image set and are ideal for the 3D rendering functions included with the CELLscan System. Importantly, only Scanalytic's EPR algorithm has been proven to retain the quantitative accuracy of the image set, permitting accurate studies on intensity level to be conducted on highly-resolved 3D images.
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